F'lacZ+cI857 (ATCC® 37446™)

Applications: expression vector  /  Depositors: M Mieschendahl

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations F'lacZ+cI857
Depositors M Mieschendahl
Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: F'lacZ+cI857 (plasmid)
Promoters: Promoter lambda PL
Marker(s):nalR
Construct size (kb): 0.0
Features: insert detection: lacZ'
marker(s): nalR
promoter: lambda PL, lambda PR
replicon: F
repressor gene: cI857
enhancer: none
Applications
expression vector
Comments
Facilitates construction and regulation of lambda promoter-dependent expression systems without using defective prophages.
Media ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
References

Mieschendahl M, Muller-Hill B. F'-coded, temperature-sensitive alpha cI857 repressor gene for easy construction and regulation of alpha promoter-dependent expression systems. J. Bacteriol. 164: 1366-1369, 1985. PubMed: 2999084

Shipped FD
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet