F'lacZ+cI857 (ATCC® 37446)

Applications: expression vector  /  Depositors: M Mieschendahl

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Designations F'lacZ+cI857
Depositors M Mieschendahl
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 0E-16
Vector: F'lacZ+cI857 (plasmid)
Promoters: Promoter lambda PL
Marker(s):nalR
Construct size (kb): 0.0
Features: insert detection: lacZ'
marker(s): nalR
promoter: lambda PL, lambda PR
replicon: F
repressor gene: cI857
enhancer: none
Applications
expression vector
Comments
Facilitates construction and regulation of lambda promoter-dependent expression systems without using defective prophages.
Media ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
References

Mieschendahl M, Muller-Hill B. F'-coded, temperature-sensitive alpha cI857 repressor gene for easy construction and regulation of alpha promoter-dependent expression systems. J. Bacteriol. 164: 1366-1369, 1985. PubMed: 2999084

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet