F'lacZ+cI857 (ATCC® 37446)

Applications: expression vector  /  Depositors: M Mieschendahl

Permits and Restrictions

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Designations F'lacZ+cI857
Depositors M Mieschendahl
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 0E-16
Vector: F'lacZ+cI857 (plasmid)
Promoters: Promoter lambda PL
Construct size (kb): 0.0
Features: insert detection: lacZ'
marker(s): nalR
promoter: lambda PL, lambda PR
replicon: F
repressor gene: cI857
enhancer: none
expression vector
Facilitates construction and regulation of lambda promoter-dependent expression systems without using defective prophages.
Media ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C

Mieschendahl M, Muller-Hill B. F'-coded, temperature-sensitive alpha cI857 repressor gene for easy construction and regulation of alpha promoter-dependent expression systems. J. Bacteriol. 164: 1366-1369, 1985. PubMed: 2999084

Shipped freeze-dried