pEMBLYe31 (ATCC® 37405)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: G Cesareni

Permits and Restrictions

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Designations pEMBLYe31
Depositors G Cesareni
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.1000003814697270
Vector: pEMBLYe31 (phagemid)
Construction: pEMBL9, (ATCC 37395), bacteriophage f1, fragment of CV13
Marker(s):LEU2,ampR
Construct size (kb): 8.100000381469727
Features: insert detection: lacZ'
marker(s): ampR, LEU2
replicon: pMB1, f1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
ssDNA-producing plasmid with polylinker in lacZ' and marker selectable in yeast.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771

Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet