pSS25 (ATCC® 37392)

Applications: vector for constructing cDNA librariesvector permitting production of single-stranded DNA  /  Depositors: J Kowalski

Permits and Restrictions

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Designations pSS25
Depositors J Kowalski
Biosafety Level 1
Vector Information
Size (kb): 3.5999999046325680
Vector: pSS25 (phagemid)
Construction: pSS34
Marker(s):ampR
Construct size (kb): 3.599999904632568
Features: marker(s): ampR
replicon: pMB1, f1
terminator: none
Applications
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
Comments
Plasmid vector for construction of cDNA libraries.
The f1 intergenic region for packaging of ssDNA was inserted into an Okayama & Berg vector. SV40 sequences have been removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Kowalski J, et al. Vectors for the direct selection of cDNA clones corresponding to mammalian cell mRNA of low abundance. Gene 35: 45-54, 1985. PubMed: 2863196

Shipped freeze-dried
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Product Sheet
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