lambdaSE4 (ATCC® 37387)

Applications: vector permitting positive selection for insertsvector with low copy number  /  Depositors: SJ Elledge

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Designations lambdaSE4
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 46.0000000000000000
Vector: lambdaSE4 (phagemid)
Construction: lambda1059, pDPT427
Marker(s):ampR,spcR,strR
Construct size (kb): 46.0
Features: insert detection: Spi+
marker(s): ampR, spcR, strR
replicon: pMB1, NR1, lambda
repressor gene: cI857
Applications
vector permitting positive selection for inserts
vector with low copy number
Comments
Plasmid is temperature inducible to produce infectious phage particles.
This phagemid grows lytically as phage in non-immune hosts or lysogenically as a phagemid in an immune host.
A phagemid vector useful for identification of genes by complementation.
With a BamHI digest, the left arm is 24.1 kb, the stuffer fragment encoding the Spi+ phenotype is 16 kb, and the right arm is 8.6 kb.
This vector uses Spi- selection for insertion of DNA into the vector and has the ability to accept 2 - 19 kb Sau3AI, BamHI, BglII, BclI, or XhoII fragments.
This vector was constructed by joining the 4.7 kb BamHI-BglII fragment of pDPT427 (low copy number replicon + spectinomycin resistance) to the left arm of lambda1059.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547

Shipped freeze-dried
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