pRB273 (ATCC® 37340)

Applications: shuttle vector  /  Depositors: R Bruckner

Permits and Restrictions

View Permits

Designations pRB273
Depositors R Bruckner
Biosafety Level 1
Vector Information
Size (kb): 5.5999999046325680
Vector: pRB273 (plasmid)
Construction: pBR322, pRB103
Marker(s):ampR,kanR
Construct size (kb): 5.599999904632568
Features: marker(s): ampR, kanR
replicon: pMB1, pUB110
enhancer: none
Applications
shuttle vector
Comments
A shuttle vector which confers both ampicillin and kanamycin resistance in Escherichia coli but only kanamycin resistance in Bacillus subtilis.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bruckner R, et al. Expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in Gram-positive and Gram-negative bacteria. Gene 32: 151-160, 1984. PubMed: 6442250

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet