pV69 (ATCC® 37313)

Applications: shuttle vector  /  Depositors: F Cuzin, G Meneguzzi

Permits and Restrictions

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Designations pV69
Depositors F Cuzin, G Meneguzzi
Biosafety Level 1
Vector Information
Size (kb): 9.3999996185302730
Vector: pV69 (plasmid)
Promoters: Promoter HSV1
Construction: pBR322, BPV-1, Tn5
Marker(s):G418R,ampR,kanR,neoR
Construct size (kb): 9.399999618530273
Features: marker(s): ampR, kanR, neoR, G418R
promoter: HSV1
replicon: pMB1, BPV1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.4.
One of the BamHI sites appears to have been lost.
Shuttle vector which can be selected for in animal cells by G418 resistance.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Meneguzzi G, et al. Plasmidial maintenance in rodent fibroblasts of a BPV1-pBR322 shuttle vector without immediately apparent oncogenic transformation of the recipient cells. EMBO J. 3: 365-371, 1984. PubMed: 6325168

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet