pV69 (ATCC® 37313)

Applications: shuttle vector  /  Depositors: F Cuzin, G Meneguzzi

Permits and Restrictions

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Designations pV69
Depositors F Cuzin, G Meneguzzi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.3999996185302730
Vector: pV69 (plasmid)
Promoters: Promoter HSV1
Construction: pBR322, BPV-1, Tn5
Construct size (kb): 9.399999618530273
Features: marker(s): ampR, kanR, neoR, G418R
promoter: HSV1
replicon: pMB1, BPV1
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--9.4.
One of the BamHI sites appears to have been lost.
Shuttle vector which can be selected for in animal cells by G418 resistance.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Meneguzzi G, et al. Plasmidial maintenance in rodent fibroblasts of a BPV1-pBR322 shuttle vector without immediately apparent oncogenic transformation of the recipient cells. EMBO J. 3: 365-371, 1984. PubMed: 6325168

Shipped freeze-dried