pTRP56 (ATCC® 37306)

Applications: YR-type (replicating) shuttle vectorexpression vectorshuttle vector  /  Depositors: A Miyajima

Permits and Restrictions

View Permits

Designations pTRP56
Depositors A Miyajima
Biosafety Level 1
Vector Information
Size (kb): 6.1999998092651370
Vector: pTRP56 (plasmid)
Promoters: Promoter ADC1
Construction: YRp7, Saccharomyces cerevisiae TRP5 terminator
Marker(s):TRP1,ampR,tetR
Construct size (kb): 6.199999809265137
Features: marker(s): ampR, tetR, TRP1
promoter: ADC1
replicon: pMB1, ARS1
terminator: TRP5
enhancer: none
Applications
YR-type (replicating) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--6.2; BamHI--6.2.
A cloning vector that uses the plasmid primer approach of Okayama & Berg to allow expression of cDNA clones in yeast.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Miyajima A, et al. Analysis of full-length cDNA clones carrying GAL1 of Saccharomyces cerevisiae: a model system for cDNA expression. Nucleic Acids Res. 12: 6397-6414, 1984. PubMed: 6206472

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet