pKH4 (ATCC® 37292)

Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: T Tsurimoto

Permits and Restrictions

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Designations pKH4
Depositors T Tsurimoto
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.5999999046325680
Vector: pKH4 (plasmid)
Promoters: Promoter recA
Construction: pBR322, pTM-2
Marker(s):ampR
Construct size (kb): 4.599999904632568
Features: marker(s): ampR
promoter: recA
replicon: pMB1
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Creates a fusion protein including the first three amino acid residues of the recA protein.
Expression vector regulated by nalidixic acid induction of the recA promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Shirakawa M, et al. Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoter-operator of Escherichia coli. Gene 28: 127-132, 1984. PubMed: 6329913

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet