pLA7 (ATCC® 37281)

Applications: vector permitting positive selection for inserts  /  Depositors: IR Beacham

Permits and Restrictions

View Permits

Designations pLA7
Depositors IR Beacham
Biosafety Level 1
Vector Information
Size (kb): 4.0999999046325680
Vector: pLA7 (plasmid)
Construction: pCB1, pBR322
Marker(s):ampR,ush
Construct size (kb): 4.099999904632568
Features: insert detection: ush+
marker(s): ampR, ush
replicon: pMB1
Applications
vector permitting positive selection for inserts
Comments
Propagation in a dam- host is necessary for cleavage at the BclI site.
A positive selection vector for cloning Sau3AI-generated DNA fragments.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burns DM, Beacham IR. Positive selector vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. Gene 27: 323-325, 1984. PubMed: 6329908

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet