lambda EMBL3 (ATCC® 37266)

Applications: vector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: H Lehrach

Permits and Restrictions

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Designations lambda EMBL3
Depositors H Lehrach
Biosafety Level 1
Vector Information
Size (kb): 44.0000000000000000
Vector: lambda EMBL3 (phage, lambda - replacement)
Construction: lambda1059, EMBL2
Construct size (kb): 44.0
Features: insert detection: Spi+
replicon: lambda
Applications
vector for constructing genomic libraries
vector permitting positive selection for inserts
Comments
Phage with inserts have the Spi- phenotype.
A lambda replacement vector for construction and screening of genomic libraries.
The left arm is 20 kb, the stuffer is 14 kb, and the right arm is 9 kb. lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in the orientation of the MCS surrounding the stuffer fragment.
References

Frischauf AM, et al. Lambda replacement vectors carrying polylinker sequences. J. Mol. Biol. 170: 827-842, 1983. PubMed: 6315951

Shipped freeze-dried
Shipping Information Distributed: freeze-dried bacteria-free lysate
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