pKT240 [CB696] (ATCC® 37258)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: M Bagdasarian

Permits and Restrictions

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Designations pKT240 [CB696]
Depositors M Bagdasarian
Biosafety Level 1
Vector Information
Size (kb): 12.8999996185302700
Vector: pKT240 (plasmid)
Promoters: Promoter none
Construction: RSF1010, pHSG415
Marker(s):ampR,kanR
Construct size (kb): 12.89999961853027
Features: insert detection: aph
marker(s): ampR, kanR
promoter: none
replicon: R300B
Applications
promoter-cloning vector
shuttle vector
Comments
This is a promoter-cloning vector containing the promoterless structural gene for aminoglycoside phosphotransferase (aph). Inserts at the EcoRI site containing promoters can be identified by streptomycin resistance.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bagdasarian MM, et al. Activity of the hybrid trp-lac(tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26: 273-282, 1983. PubMed: 6323265

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37258