pKT240 [CB696] (ATCC® 37258)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: M Bagdasarian

Permits and Restrictions

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Designations pKT240 [CB696]
Depositors M Bagdasarian
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 12.8999996185302700
Vector: pKT240 (plasmid)
Promoters: Promoter none
Construction: RSF1010, pHSG415
Construct size (kb): 12.89999961853027
Features: insert detection: aph
marker(s): ampR, kanR
promoter: none
replicon: R300B
promoter-cloning vector
shuttle vector
This is a promoter-cloning vector containing the promoterless structural gene for aminoglycoside phosphotransferase (aph). Inserts at the EcoRI site containing promoters can be identified by streptomycin resistance.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Bagdasarian MM, et al. Activity of the hybrid trp-lac(tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26: 273-282, 1983. PubMed: 6323265

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37258