pUC18 (ATCC® 37253)

Other IDs:

Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand).

Nucleotide (GenBank) : VB0025 pUC18 cloning vector.

 /  Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: J Messing

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Designations pUC18
Other IDs

Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand).

Nucleotide (GenBank) : VB0025 pUC18 cloning vector.

Depositors J Messing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.686
Vector: pUC18 (plasmid)
Promoters: Promoter lac
Construction: pUC71K
Marker(s):ampR
Construct size (kb): 2.686
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879

Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37253