pUC18 (ATCC® 37253)

Other IDs:

Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand).

Nucleotide (GenBank) : VB0025 pUC18 cloning vector.

 /  Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: J Messing

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Designations pUC18
Depositors J Messing
Other IDs

Nucleotide (GenBank) : VB0032 pUC18c cloning vector (beta-galactosidase mRNA on complementary strand).

Nucleotide (GenBank) : VB0025 pUC18 cloning vector.

Biosafety Level 1
Vector Information
Size (kb): 2.686
Vector: pUC18 (plasmid)
Promoters: Promoter lac
Construction: pUC71K
Marker(s):ampR
Construct size (kb): 2.686
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879

Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried plasmid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37253