pUC9 (ATCC® 37252)

Other IDs:

Nucleotide (GenBank) : VB0022 pUC9 cloning vector.

Nucleotide (GenBank) : VB0029 pUC9c cloning vector (beta-galactosidase mRNA on complementary strand).

 /  Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: J Messing

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Designations pUC9
Depositors J Messing
Other IDs

Nucleotide (GenBank) : VB0022 pUC9 cloning vector.

Nucleotide (GenBank) : VB0029 pUC9c cloning vector (beta-galactosidase mRNA on complementary strand).

Biosafety Level 1
Vector Information
Size (kb): 2.7000000476837160
Vector: pUC9 (plasmid)
Promoters: Promoter lac
Construction: pUC71K
Marker(s):ampR
Construct size (kb): 2.700000047683716
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879

Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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