pJKK3-1 (ATCC® 37220)

Applications: shuttle vector  /  Depositors: J Kreft

Permits and Restrictions

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Designations pJKK3-1
Depositors J Kreft
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.4000000953674320
Vector: pJKK3-1 (plasmid)
Marker(s):tetR
Construct size (kb): 7.400000095367432
Features: marker(s): tetR
replicon: pMB1, pBC16
Applications
shuttle vector
Comments
A Bacillus subtilis/Escherichia coli shuttle vector.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Kreft J, et al. Cloning and expression in Escherichia coli and Bacillus subtilis of the hemolysin (cereolysin) determinant from Bacillus cereus. J. Bacteriol. 155: 681-689, 1983. PubMed: 6307975

Kreft J, Hughes C. Cloning vectors derived from plasmids and phage of Bacillus. Curr. Top. Microbiol. Immunol. 96: 1-17, 1982. PubMed: 6276085

Shipped freeze-dried
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