pY2 (ATCC® 37186)

Applications: expression vector  /  Depositors: JJ Rossi

Permits and Restrictions

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Designations pY2
Depositors JJ Rossi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.6999998092651370
Vector: pY2 (plasmid)
Promoters: Promoter synthetic
Construction: pBR327
Construct size (kb): 4.699999809265137
Features: marker(s): ampR, cmlR, tetR
promoter: synthetic
replicon: pMB1
expression vector
Contains a synthetic consensus Escherichia coli RNA polymerase promoter, HindIII CAT gene cartridge, and pBR327 amp and tet resistance genes.
Identical to pXJ002 (ATCC 37183) except the transcription start site is altered from CCC to CAT.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698

Shipped freeze-dried