pYEJ001 (ATCC® 37185)

Applications: expression vector  /  Depositors: JJ Rossi

Permits and Restrictions

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Designations pYEJ001
Depositors JJ Rossi
Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: pYEJ001 (plasmid)
Promoters: Promoter synthetic
Construction: pBR327
Marker(s):ampR,cmlR,tetR
Construct size (kb): 0.0
Features: marker(s): ampR, cmlR, tetR
promoter: synthetic
replicon: pMB1
Applications
expression vector
Comments
An expression vector containing a synthetic consensus Escherichia coli RNA polymerase promoter, tandem synthetic lactose operators, a HindIII CAT gene cartridge as well as the pBR327 amp and tet resistance genes.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet