pYEJ001 (ATCC® 37185)

Applications: expression vector  /  Depositors: JJ Rossi

Permits and Restrictions

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Designations pYEJ001
Depositors JJ Rossi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 0E-16
Vector: pYEJ001 (plasmid)
Promoters: Promoter synthetic
Construction: pBR327
Marker(s):ampR,cmlR,tetR
Construct size (kb): 0.0
Features: marker(s): ampR, cmlR, tetR
promoter: synthetic
replicon: pMB1
Applications
expression vector
Comments
An expression vector containing a synthetic consensus Escherichia coli RNA polymerase promoter, tandem synthetic lactose operators, a HindIII CAT gene cartridge as well as the pBR327 amp and tet resistance genes.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet