pEA300 (ATCC® 37181)

Applications: contains easily purifiable cassette(s) for constructionexpression vector  /  Depositors: E Amann

Permits and Restrictions

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Designations pEA300
Depositors E Amann
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pEA300 (plasmid)
Promoters: Promoter trp
Construction: pKK84-1, ptrpH1
Marker(s):ampR
Construct size (kb): 5.5
Features: marker(s): ampR
promoter: trp
replicon: pMB1
transcription terminator: rrnB
Applications
contains easily purifiable cassette(s) for construction
expression vector
Comments
Constructed by inserting a 192 bp fragment containing the -35 sequence of the trp promoter into the ClaI site of pKK84-1.
Plasmid contains two terminators tandemly arranged.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212

Shipped freeze-dried
Product Sheet
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