pEA300 (ATCC® 37181)

Applications: contains easily purifiable cassette(s) for constructionexpression vector  /  Depositors: E Amann

Permits and Restrictions

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Designations pEA300
Depositors E Amann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5000000000000000
Vector: pEA300 (plasmid)
Promoters: Promoter trp
Construction: pKK84-1, ptrpH1
Construct size (kb): 5.5
Features: marker(s): ampR
promoter: trp
replicon: pMB1
transcription terminator: rrnB
contains easily purifiable cassette(s) for construction
expression vector
Constructed by inserting a 192 bp fragment containing the -35 sequence of the trp promoter into the ClaI site of pKK84-1.
Plasmid contains two terminators tandemly arranged.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Amann E, et al. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178, 1983. PubMed: 6363212

Shipped freeze-dried