pLV57 (ATCC® 37177)

Applications: vector permitting positive selection for inserts  /  Depositors: D O'Connor

Permits and Restrictions

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Designations pLV57
Depositors D O'Connor
Biosafety Level 1
Vector Information
Size (kb): 6.0999999046325680
Vector: pLV57 (plasmid)
Construction: pBR325, NTP14m07(ts)
Marker(s):ampR,cmlR,EcoRIm(ts)r+
Construct size (kb): 6.099999904632568
Features: insert detection: R+Mts
marker(s): ampR, cmlR, EcoRIm(ts)r+
replicon: pMB1
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): HincII--6.2; HindIII/BamHI--6.2; BamHI--uncut.
Positive selection cloning vector employing EcoRI restriction-modification system.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

O'Connor CD, Humphreys GO. Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors. Gene 20: 219-229, 1982. PubMed: 6299889

Shipped freeze-dried
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