pLV57 (ATCC® 37177)

Applications: vector permitting positive selection for inserts  /  Depositors: D O'Connor

Permits and Restrictions

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Designations pLV57
Depositors D O'Connor
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.0999999046325680
Vector: pLV57 (plasmid)
Construction: pBR325, NTP14m07(ts)
Marker(s):ampR,cmlR,EcoRIm(ts)r+
Construct size (kb): 6.099999904632568
Features: insert detection: R+Mts
marker(s): ampR, cmlR, EcoRIm(ts)r+
replicon: pMB1
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): HincII--6.2; HindIII/BamHI--6.2; BamHI--uncut.
Positive selection cloning vector employing EcoRI restriction-modification system.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

O'Connor CD, Humphreys GO. Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors. Gene 20: 219-229, 1982. PubMed: 6299889

Shipped freeze-dried
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