pSV3-dhfr (ATCC® 37147)

Applications: expression vectorshuttle vector  /  Depositors: P Berg

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Designations pSV3-dhfr
Depositors P Berg
Biosafety Level 1
Vector Information
Size (kb): 8.4
Vector: pSV3-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse
Marker(s):ampR,mtxR
Construct size (kb): 8.4
Features: marker(s): ampR, mtxR
other: SV40 large T antigen
promoter: SV40
replicon: pMB1
Applications
expression vector
shuttle vector
Comments
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture cells.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37147