pKO11 (ATCC® 37122)

Applications: promoter-cloning vector  /  Depositors: M Rosenberg

Permits and Restrictions

View Permits

Designations pKO11
Depositors M Rosenberg
Biosafety Level 1
Vector Information
Size (kb): 3.9000000953674320
Vector: pKO11 (plasmid)
Promoters: Promoter none
Construction: pKO1 (ATCC 37126)
Marker(s):ampR
Construct size (kb): 3.900000095367432
Features: insert detection: galK
marker(s): ampR
promoter: none
replicon: pMB1
Applications
promoter-cloning vector
Comments
This is a promoter cloning vector using expression of the promoterless galK gene for positive or color (MacConkey galactose indicator plates) selection.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056

Adams CW, Hatfield GW. Effects of promoter strengths and growth conditions on copy number of transcriptions-fusion vectors. J. Biol. Chem. 259: 7399-7403, 1984. PubMed: 6736011

Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet