YIp30 [pRB8] (ATCC® 37109)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp30 [pRB8]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: YIp30 (plasmid)
Construction: pBR322, Saccharomyces cerevisiae
Marker(s):URA3,ampR
Construct size (kb): 5.5
Features: marker(s): ampR, URA3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
HV2 vector when used with S. cerevisiae SHY series hosts (ATCC 44769 - 44772).
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 into the HindIII site of pBR322. The 5' - 3' orientation of URA3 is counterclockwise, with the SmaI site in 3' URA3 clone to the EcoRI site of pBR322.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet