pMOB48 (ATCC® 37107)

Applications: expression vector  /  Depositors: D Vapnek

Permits and Restrictions

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Designations pMOB48
Depositors D Vapnek
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.5000000000000000
Vector: pMOB48 (plasmid)
Promoters: Promoter lacUV5
Construction: R1, M13mp7
Marker(s):cmlR
Construct size (kb): 9.5
Features: insert detection: lacZ'
marker(s): cmlR
promoter: lacUV5
replicon: R1(ts)
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI--7.2, 2.3; HindIII--8.5, 1.0.
An expression vector with copy number amplifiable by heat induction without protein synthesis inhibition. Can be used for lacIq-controlled expression of proteins.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 30.0°C
References

Bittner M, Vapnek D. Versatile cloning vectors derived from the runaway-replication plasmid pKN402. Gene 15: 319-329, 1981. PubMed: 6277736

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet