pBD64 (ATCC® 37104)

Applications: shuttle vector  /  Depositors: D Dubnau

Permits and Restrictions

View Permits

Designations pBD64
Depositors D Dubnau
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.6999998092651370
Vector: pBD64 (plasmid)
Construction: pUB110, pC194
Construct size (kb): 4.699999809265137
Features: marker(s): cmlR, kanR
replicon: pUB110
shuttle vector
A general purpose plasmid vector for cloning in Bacillus subtilis.
This vector was isolated as a spontaneous deletion variant of pBD12, a chimeric vector composed of plasmids pUB110 and pC194. The deletion consists of 1844 bp (nt 3803 to 5649) of pBD12, and encompasses nt 1 to 487 and 1552 to 2906 of the pC194 sequence.
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 37.0°C

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Minton NP, et al. The gram-positive cloning vector pBD64 arose by a 1844 bp deletion of pC194 derived DNA. Nucleic Acids Res. 18: 1651, 1990. PubMed: 2326208

Shipped freeze-dried