pNO1517 (ATCC® 37094)

Applications: vector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: D Dean

Permits and Restrictions

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Designations pNO1517
Depositors D Dean
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.1999998092651370
Vector: pNO1517 (cosmid)
Promoters: Promoter str
Construction: pBR322, lambda fus3
Construct size (kb): 8.199999809265137
Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1
vector for constructing genomic libraries
vector permitting positive selection for inserts
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A cosmid vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C

Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634

Shipped freeze-dried