pNO1517 (ATCC® 37094)

Applications: vector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: D Dean

Permits and Restrictions

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Designations pNO1517
Depositors D Dean
Biosafety Level 1
Vector Information
Size (kb): 8.1999998092651370
Vector: pNO1517 (cosmid)
Promoters: Promoter str
Construction: pBR322, lambda fus3
Marker(s):ampR
Construct size (kb): 8.199999809265137
Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1
Applications
vector for constructing genomic libraries
vector permitting positive selection for inserts
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A cosmid vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634

Shipped freeze-dried
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