pS194 (ATCC® 37082)

Applications: shuttle vector  /  Depositors: L Philipson

Permits and Restrictions

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Designations pS194
Depositors L Philipson
Biosafety Level 2
Vector Information
Size (kb): 4.3969998359680180
Vector: pS194 (plasmid)
Construction: natural isolate
Marker(s):strR
Construct size (kb): 4.396999835968018
Features: marker(s): strR
replicon: pS174
Applications
shuttle vector
Comments
Contains one EcoRI and one HindIII site and no sites for BamHI or SmaI.
natural isolate
Vector for construction and propagation of hybrid DNA in Staphylococcus aureus or Bacillus subtilis.
Media ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 30.0°C
References

Lofdahl S, et al. Characterization of small plasmids from Staphylococcus aureus. Gene 3: 145-149, 1978. PubMed: 95989

Projan SJ, et al. Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus. Nucleic Acids Res. 16: 2179-2187, 1988. PubMed: 3357770

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of Staphylococcus aureus containing the plasmid.

Product Sheet
Product Sheet
Product Sheet