YIp26 [pRB9] (ATCC® 37057)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp26 [pRB9]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 7.8000001907348630
Vector: YIp26 (plasmid)
Construction: pBR322, Saccharomyces cerevisiae
Marker(s):LEU2,URA3,ampR
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, LEU2, URA3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
Orientations are such that transcription from both URA3 and LEU2 is counterclockwise.
A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. The unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
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