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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 7.7690000534057620 Vector: YEp24 (plasmid) Promoters: Promoter none Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori Marker(s):URA3,ampR,tetR Construct size (kb): 7.769000053405762 Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none
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Applications
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YE-type (episomal) shuttle vector shuttle vector vector for constructing genomic libraries
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Comments
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YE-type episomal shuttle vector. Plasmid was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites of pBR322.
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References
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Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
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Shipping Information
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Shipped: Frozen glycerol stock of E. coli containing the plasmid.
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