YEp24 [pRB5] (ATCC® 37051)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: D Botstein

Permits and Restrictions

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Designations YEp24 [pRB5]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.7690000534057620
Vector: YEp24 (plasmid)
Promoters: Promoter none
Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori
Marker(s):URA3,ampR,tetR
Construct size (kb): 7.769000053405762
Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
YE-type episomal shuttle vector.
Plasmid was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Related Products
component of:ATCC 87635
Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37051