pKT279 (ATCC® 37035)

Applications: expression vectorvector with sequences for protein export  /  Depositors: WD Gilbert

Permits and Restrictions

View Permits

Designations pKT279
Depositors WD Gilbert
Biosafety Level 1
Vector Information
Size (kb): 3.7999999523162840
Vector: pKT279 (plasmid)
Promoters: Promoter amp
Construction: pBR322
Marker(s):tetR
Construct size (kb): 3.799999952316284
Features: marker(s): tetR
promoter: amp
replicon: pMB1
terminator: none
enhancer: none
Applications
expression vector
vector with sequences for protein export
Comments
PstI cloning site distal to pre-penicillinase signal sequence.
Vector for secretion of cloned gene product.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Talmadge K, Gilberg W. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region. Gene 12: 235-241, 1980. PubMed: 6265317

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet