pGH6 (ATCC® 31539)

Applications: expression vector  /  Depositors: Genentech, Inc.

Permits and Restrictions

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Designations pGH6
Depositors Genentech, Inc.
Biosafety Level 1
Host
Distribution host: Escherichia coli 294; GMBH K-12 294
Distribution host: Escherichia coli 294; GMBH K-12 294
Vector Information
Size (kb): 4.5999999046325680
Vector: pGH6 (plasmid)
Promoters: Promoter lacUV5
Construction: pBR322, pKB268
Marker(s):ampR,tetR
Construct size (kb): 4.599999904632568
Features: marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Applications
expression vector
Comments
pGH6 is available in a bacteria culture as ATCC 31539 or as purified plasmid DNA as ATCC 40012.
Expression vector. The EcoRI site is downstream of the lacUV5 promoters.
This was constructed by inserting a 285 bp EcoRI fragment from pKB268 (containing 2 95 bp lac UV5 promoters separated by heterologous DNA) into the EcoRI site of pBR322. The EcoRI site distal to the tetR gene was destroyed.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet