Related Products S. cerevisiae/E. coli marker swap vectors (ATCC® 87561™) ATCC® Number: 87561 ™ Storage: Store frozen plate at -800 C. Applications: A set of marker swap vectors, containing gene disruptions for standard markers such as his3::LEU2, useful in changing markers in the S. cerevisiae host chromosome or on plasmids. To convert the host phenotype from the existing yeast auxotrophic marker to a new marker (eg. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. For-Profit: $290.00 Non-Profit: $290.00 Add to Cart Preview Product Compare Product Format: frozen Comments: Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates. In most but not all cases the frequencies of successful convertants are greater than 30%. A set of marker swap vectors, containing gene disruptions for standard markers such as his3::LEU2, useful in changing markers in the S. cerevisiae host chromosome or on plasmids. The set allows interconversion among all six of the common markers HIS3, LEU2, TRP1, URA3, ADE2, and LYS2. To convert the host phenotype from the existing yeast auxotrophic marker to a new marker (eg. HIS3 --> LEU2), transform with the restriction enzyme digested vector (eg. ApaI+PstI digested pHL3) and select for the appropriate phenotype (for pHL3, Leu+). When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Product Sheet Certificateof Analysis MSDS Water, Cell Culture Grade (ATCC® 30-2205™) ATCC® Number: 30-2205 ™ Quantity: 500 mL Storage: Store cell culture grade water at room temperature (15°C to 30°C). Shelf life of 36 months when properly stored. Applications: For use as a diluent or for reconstituting powdered media and salt solutions. For-Profit: $10.00 Non-Profit: $10.00 Add to Cart Preview Product Compare Product Format: 500 mL Comments: Cell culture tested. Product Sheet Certificateof Analysis MSDS S. cerevisiae/E. coli pRS400 shuttle vectors (ATCC® 87538™) ATCC® Number: 87538 ™ Depositor: P Hieter, JD Boeke, TW Christianson For-Profit: $3,850.00 Non-Profit: $3,850.00 Add to Cart Preview Product Compare Product Sheet Certificateof Analysis MSDS Molecular Grade Water (ATCC® 60-2450™) ATCC® Number: 60-2450 ™ Quantity: 1 liter Storage: Store at 15°C to 30°C Applications: For use in all molecular biology procedures that require water, including PCR. Distilled, deionized, sterile-filtered. For-Profit: $25.00 Non-Profit: $25.00 Add to Cart Preview Product Compare Product Format: 1 liter Product Sheet Certificateof Analysis MSDS SRS for Detecting Protein-Protein Interactions (ATCC® 87639™) ATCC® Number: 87639 ™ Depositor: SJ Elledge For-Profit: $290.00 Non-Profit: $290.00 Add to Cart Preview Product Compare Product Sheet Certificateof Analysis MSDS 384-Well Plate Seals (ATCC® 60-2410™) ATCC® Number: 60-2410 ™ Quantity: Box of 100 seals Storage: 15 to 30oC Applications: Foil seals are used for sample storage and manipulation on microplates. For-Profit: $130.00 Non-Profit: $130.00 Add to Cart Preview Product Compare Product Format: Box of 100 seals Comments: Foil seals are also available in 96-well format. Patent pending. Product Sheet Certificateof Analysis MSDS LB Broth (ATCC® 60-2100™) ATCC® Number: 60-2100 ™ Quantity: 1 liter Storage: 15°C to 30°C (ambient temperature) Applications: Sterile liquid is ready to use.Luria-Bertani medium for propagation of bacteria for molecular biology applications. For-Profit: $30.00 Non-Profit: $30.00 Add to Cart Preview Product Compare Product Format: 1 liter Product Sheet Certificateof Analysis MSDS pJD Plasmid Control Series for Mutation Analysis (ATCC® 87584™) ATCC® Number: 87584 ™ Applications: A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning.These plasmids are designed so that the same PCR primer pair can be used on each plasmid. For-Profit: $345.00 Non-Profit: $345.00 Add to Cart Preview Product Compare Product Format: freeze-dried Comments: Primers have been tested that yield DNA fragments sizes ranging between 203 and 1455 bp.A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning. The mutations are either point variants (A, T, C or G at nt 272) or insertions of one to five nucleotides (starting at nt 274).The plasmids were derived from pUC19 (GenBank M77789), with each plasmid containing a 10 to 15 base pair replacement between the BamHI and EcoRI sites. These plasmids are designed so that the same PCR primer pair can be used on each plasmid. Product Sheet Certificateof Analysis MSDS 1