Prototrophic for purines, pyrimidines, Ala, Asn, Asp, Gln, Lys, Phe, Pro, and Tyr; auxotrophic for His, Ile, Met, and Trp. Growth is poor without Arg, Glu, Gly, Leu, and Val.
Host strain with well-defined restriction maps for SfiI, NotI, and AscI.
Phenotype verified: rifR.
Derived from E. faecalis OG1 by selection for resistance to rifampin and fusidic acid.