Escherichia coli (Migula) Castellani and Chalmers (ATCC® 33572)

Strain Designations: LE392 [NRRL B-14219]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations LE392 [NRRL B-14219]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- supE44 supF58 lacY1 or deltalac(I-Y)6 galK2 galT22 metB1 trpR55 hsdR514 mcrB lambda-
Type Strain no
Comments
A host for plating lambda libraries or clones requiring suppression of amber mutations (i.e. Charon 4A, Charon 21A, etc.). Strain LE392 same as NRRL B-14219.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- supE44 supF58 lacY1 or deltalac(I-Y)6 galK2 galT22 metB1 trpR55 hsdR514 mcrB lambda-
Name of Depositor P Leder
References

Murray NE, et al. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol. Gen. Genet. 150: 53-61, 1977. PubMed: 319344

Leder P, et al. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher-organisms: the lambdagtWES system. Science 196: 175-177, 1977. PubMed: 322278

Brunel F, et al. Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage lambdagtWES. Gene 8: 53-68, 1979. PubMed: 535730

Gowda K, et al. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene 207: 197-207, 1998. PubMed: 9511762