pACYC177/ET3d/yNMT (ATCC® 87052)

Other IDs:

Nucleotide (GenBank) : U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.

 /  Applications: produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase  /  Depositors: RS Haun

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Designations pACYC177/ET3d/yNMT
Depositors RS Haun
Other IDs

Nucleotide (GenBank) : U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.

Biosafety Level 1
Host
Distribution host: Escherichia coli BL21(DE3)
Vector Information
Size (kb): 4.9429998397827150
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC177/ET3d
Intact vector size:
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI, NcoI+BamHI
Polylinker sites:
Construction: pACYC177,pET3d
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: p15A
marker(s): kanR, ->
ribosome-binding site: gene 10
promoter: T7 (phi10), ->
Cross references:
Vector: pACYC177/ET3d/yNMT (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pACYC177,pET3d
Marker(s):kanR
Construct size (kb): 4.900000095367432
Features: marker(s): kanR
promoter for expression: T7 (phi10)
replicon: p15A
coding sequence: NMT1
Applications
produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase
Comments
Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5.
Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.
Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4.
Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Haun RS, et al. Effect of myristoylation on GTP-dependent binding of ADP-ribosylation factor to Golgi. J. Biol. Chem. 268: 7064-7068, 1993. PubMed: 8463239

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
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