c8543 (ATCC® 71210)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: L Riles, MV Olson

Permits and Restrictions

View Permits

Designations c8543
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors L Riles, MV Olson
Vector
Construct size (kb): 45.29999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: CDC3
Contains complete coding sequence?: Y
Chromosome: XII; Localization: XII R, 225 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: REC102
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: MID2
Gene name: mating process protein
Contains complete coding sequence?: Y
Chromosome: XII
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 37.7
Cross references:
Insert lengths(kb): 37.70000076293945
Gene product: mating process protein [CDC3]
Insert Size (kb) 37.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet