c8035 [8035] (ATCC® 70902)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Permits and Restrictions

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Designations c8035 [8035]
GenBank Number

U33050

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Applications
The ATCC encourages users of this material to communicate their results to us.
Vector
Construct size (kb): 42.58
Insert
DNA: genomic

Insert information:

Insert size (kb):  36.146

Insert ends : MboI

Genes :

                mating factor a, MFA1,

                ribosomal protein L27b, RPL27B

                negative regulator of prp genes,  SPP41

                nuclear-localized protein containing zinc finger            motifs, involved in pre-tRNA splicing, STP1

DNA : genomic, chromosome IV R

Source : Saccharomyces cerevisiae AB972 (ATCC 76269)

Genbank accession : Nucleotides 1-36146 of the insert correspond to nucleotides 2618-38763 of U33050.

Insert lengths(kb): 36.14599990844727
Gene product: mating factor a [MFA1]
Gene product:ribosomal protein L27b (RPL27B)
Gene product: negative regulator of prp genes (SPP41)
Gene product: nuclear-localized protein containing zinc finger motifs, involved in pre-tRNA splicing (STP1)
Insert Size (kb) 36.146
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Frozen glycerol stock of E. coli containing the cosmid.

Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.077, 2.207, 2.564, 2.534, 0.728, 1.488, 2.242, 0.862, 3.562, 2.528, 2.803, 0.799, 1.821, 2.002, 1.215, 0.545, 1.168, 0.406.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70439, 70457, 70572, 70736.
NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
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