c9202 [9202] (ATCC® 70890)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Designations c9202 [9202]
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Vector
Construct size (kb): 0.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene name: DNA Segment
Contains complete coding sequence?: U
Chromosome: VIII; Localization: VIII R
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references:
Gene product: DNA Segment
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 2.012, 5.306, 0.882, 0.413, 1.999, 0.976, 1.634, 1.517, 6.536, 0.538, 1.030, 1.374, 0.507, 2.434, 0.534, 6.513, 0.985, 0.730.
Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.42, 0.53, 0.73, 0.89, 1.0, 1.0, 1.4, 1.5, 1.7, 2.1, 2.5, 5.6, 6.8.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70098, 70403, 70487, 70886, 70899, 70923.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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