lambdaPM-6235 [6235] (ATCC® 70700)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

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Designations lambdaPM-6235 [6235]
GenBank Definition

Saccharomyces cerevisiae putative protein kinase (MRK1) gene,

GenBank Number


Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Construct size (kb): 0.0
Name of vector: lambdaMG3
Intact vector size:
Type of vector: phage
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: L47.1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
DNA: genomic
Genome: Saccharomyces cerevisiae
Gene symbol: MRK1
Gene name: glycogen synthase kinase-3-related
Contains complete coding sequence?: U
Chromosome: IV
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb):
Cross references:
Gene product: glycogen synthase kinase-3-related [MRK1]
Related Products
component of:ATCC 77284
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.798, 0.826, 2.116, 1.826, 2.335, 1.163, 0.704, 2.084, 0.899, 0.779, 0.905, 0.773, 0.450, 0.586, 1.134.
Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.45, 0.59, 0.64, 0.70, 0.77, 0.78, 0.82, 0.88, 0.90, 0.90, 1.1, 1.2, 1.4, 1.8, 1.8.
MRK1 occurs in a 3.9 kb EcoRI fragment, and in a 1.8 kb EcoRI/SpeI subfragment.
Overlaps clones with ATCC number(s): 70751, 70996.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Olson MV, et al. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986. PubMed: 3463999

Hardy TA, et al. Novel Saccharomyces cerevisiae gene, MRK1, encoding a putative protein kinase with similarity to mammalian glycogen synthase kinase-3 and Drosophila zeste-white/shaggy. Biochem. Biophys. Res. Commun. 208: 728-734, 1995. PubMed: 7695629

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