VMTRC49 (ATCC® 87764)

Organism: Cryptosporidium sp.  /  Clone Type: Library  /  Depositors: NV Khramtsov, SJ Upton

Permits and Restrictions

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Designations VMTRC49
Species Cryptosporidium sp.
Depositors NV Khramtsov, SJ Upton
Origin
Cryptosporidium andersoni oocysts/sporozoites
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: Cryptosporidium andersoni oocysts/sporozoites
DNA: genomic
Digest: Complete
Insert size range: 0.6-7.0 kb
Coverage: Clones for "complete" coverage: 30000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: Frozen
Comments
The VMTRC49 isolate of Cryptosporidium andersoni was collected from the feces of a Holstein cow, Bos taurus, in August, 1998 near Tulare, CA. Oocysts for DNA extraction were submitted by Dr. E.R. Atwill at University of California-Davis.
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