Cornell - 1 (ATCC® 87714)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

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Designations Cornell - 1
Species Cryptosporidium parvum Tyzzer
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: sporozoites/oocysts
DNA: genomic
Digest: Complete
Insert size range: 0.2 - 5.0
Coverage: Clones for "complete" coverage: 13000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
The Cornell - 1 isolate was collected in July, 1997 from the feces of an AIDS patient at the Cornell University Medical Center. Oocysts were purified from feces on CsCl gradients and surfaced sterilized with 10% Clorox prior to nucleic acid extraction.
References

Nikolai V Khramtsov, personal communication

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