IowaI (ATCC® 87668)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

Permits and Restrictions

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Designations IowaI
Species Cryptosporidium parvum Tyzzer
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
Tissue: sporozoites/oocysts
Number of independent recombinants: 1.7 x10(5)
Percentage non-recombinants: 15%
Titer: 3.0 x 10(8) cfu/ml
DNA: genomic
Digest: Complete
Insert size range: 0.3 - 3.0 kb
Coverage: Clones for "complete" coverage: 17000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information

 Frozen stock of library in Escherichia coli.

                   

Comments
DNA was isolated from oocysts of Cryptosporidium parvum, Iowa (I) isolate, obtained from the feces of a calf near Ames Iowa in the mid-1980's and are passaged continually in calves at Pleasant Hill Farm in Idaho.
DNA was isolated from oocysts of Cryptosporidium parvum, Iowa (I) isolate, obtained from the feces of a calf near Ames, Iowa in the mid-1980's and have been passaged continually in calves in Idaho since that time.
References

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

Slapeta J. Cryptosporidium species found in cattle: a proposal for a new species. Trends Parasitol. 22: 469-474, 2006.

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