KSU-1 (ATCC® 87439)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: NV Khramtsov, SJ Upton

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Designations KSU-1
Species Cryptosporidium parvum Tyzzer
Depositors NV Khramtsov, SJ Upton
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
DNA: genomic
Digest: Complete
Insert size range: 0.2-5.0 Kb
Coverage: Clones for "complete" coverage: 15000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
The KSU-1 isolate of cryptosporidium parvum was isolated from the feces of naturally infected calf (Bos taurus) in 1987. The isolate was passaged 6-10 times per year by oral inoculation of goats, mice and/or calves prior to library construction in 1992.
Approximately 1% of the recombinants in this library contain two ligated EcoRI inserts.
References

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

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