lambdaADH - human B cell cDNA (ATCC® 87286)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaADH - human B cell cDNA
Species Homo sapiens, human
Depositors SJ Elledge
Origin
B lymphocyte (EBV transformed)
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaADH
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI EcoRI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Cloning capacity: 0 - 8.4 kb
Construction: lambdaYES-R, pADH
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: ADH, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
Tissue: B lymphocyte (EBV transformed)
DNA: cDNA
Insert size range: 0.7 - 7 kb
Insert ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
This has the ADH promoter driving cDNAs, but require their own translational start.
Product Sheet
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