2P2 (ATCC® 87189)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

View Permits

Designations 2P2
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p23-p21
DNA: genomic
Digest: Complete
Insert size range: 50 - 600 bp
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Approximately 45% of the clones contain repetitive sequences. Of 94 single copy clones tested, 60 (64%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Kao FT, et al. A region-specific microdissection library for human chromosome 2p23-->p21 and the analysis of an interstitial deletion of 2p21. Cytogenet. Cell Genet. 68: 17-18, 1995. PubMed: 7956351

Product Sheet
Product Sheet
Product Sheet