2P4 (ATCC® 87104)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2P4
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p11-p13
DNA: genomic
Digest: Complete
Insert size range: 100-800 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Purity: 90%. Approximately 30-40% of clones contain non-repetitive sequences.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, and amplifying by PCR.
References

Yu J, et al. Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13. Somatic Cell Mol. Genet. 20: 133-136, 1994. PubMed: 8009366

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