(ATCC® 87003)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Species Homo sapiens, human
Depositors SJ Elledge
B lymphocyte
For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059).
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI Modification: T-filled
Vector end: XhoI Modification: T-filled
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Tissue: B lymphocyte
Insert size range: to 7.6 kb
Genome Equivalent: 0.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 mL)
For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059).
Constructed by ligating cDNAs to adaptors and inserting into T-filled XhoI sites of lambdaACT, as described for lambdaYES.

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

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