pFL61 Arabidopsis (ATCC® 77500)

Organism: Arabidopsis thaliana Heinhold, mouse-ear cress  /  Clone Type: Library  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL61 Arabidopsis
Species Arabidopsis thaliana Heinhold, mouse-ear cress
Depositors F Lacroute
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFL61
Intact vector size: 5.425
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Cross references:
Vector
DNA: cDNA
Insert size range: 0.3 - 3.0 kb
Genome Equivalent: 0.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
The inserts can be excised with a NotI digest.
Deposited as two cDNA banks of purified plasmid DNA.
Constructed by adding a BstXI adaptor 5'CTAAATTACTCACA-3'/3'-GATTTAATGA-5' to both ends of the cDNA.
Demonstrated to have an insert range of approximately 0.6 - 3.0 kb.
References

Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803

d'Enfert C, et al. . Methods Cell Biol. Plant Cell Biol. : submitted..

Francois Lacroute, personal communication

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