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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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glioblastoma U-138 MG cell line
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Vector
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Vector : lambda SHK Intact vector size (kb) : unknown Vector type : phage Construction : Charon BS, pSH4 Features (with orientation and position, when available) :
Marker : kanR (KmR)
Replicon : lambda, pMB1
Terminator : SV40 T antigen
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Vector
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Tissue: glioblastoma U-138 MG cell line Type of insert: cDNA Insert size range: > 0.8 kb Genome Equivalent: 0.0 Genome: human Vector: Lambda SHK Vector ends : Modification : 5' EcoRI, 3' HindIII Number of independent recombinants : 2.1 x 106 Titer : 5.0 x 107 pfu/ml
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Shipping Information
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Frozen bacteria-free phage lysate.
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Comments
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Titer determined on E. coli LE392. cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
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References
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Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057
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