GL1 (ATCC® 77440)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

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Designations GL1
Species Homo sapiens, human
Depositors A Swaroop
glioblastoma U-138 MG cell line
Vector : lambda SHK
Intact vector size (kb) : unknown
Vector type : phage
Construction : Charon BS, pSH4
Features (with orientation and position, when available) :
Marker : kanR (KmR)
Replicon : lambda, pMB1
Terminator : SV40 T antigen
Tissue: glioblastoma U-138 MG cell line
Type of insert: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Genome: human
Vector: Lambda SHK
Vector ends : Modification : 5' EcoRI, 3' HindIII
Number of independent recombinants : 2.1 x 106
Titer : 5.0 x 107 pfu/ml
Biosafety Level 1
Shipping Information Frozen bacteria-free phage lysate.
Titer determined on E. coli LE392.
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

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