EY2 [lambdaLASEY2] (ATCC® 77439)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

View Permits

Designations EY2 [lambdaLASEY2]
Species Homo sapiens, human
Depositors A Swaroop
Origin
eye cell line
Vector
Vector :Charon BS- (ATCC 77446)
Cloning capacity : < 8.9 kb
Vector type : phage
Intact vector size (kb) : 43.1
Construction : Charon 15, pBluescript KS M13(-)
Features : (with orientation and position, when available) :
Insert detection : lacZ', <--
Other : left arm : -->, 1-21225
Other : pBluescript KS - -->, 21226-24184
MCS : NotI?KpnI, --> , 21226-21315
Promoter for in vitro transcription : T3, <--, 21327-21346
Promoter : lac, <--, 21416-21444
Replicon : pMB1, 21771
Marker : ampR , <--, 22529-23389
Replicon : f1, -->, 23520-23976
Promoter for in vitro transcription : T7, -->, 24140-24159
MCS : SacI?NotI, -->, 24172-24184
Other : right arm, -->, 24185-43100
Vector
Tissue: eye, cell line CRL-7475
Type of insert: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Genome: human
Vector : Charon BS-
Insert size range (kb) : > 0.8 kb
Vector ends : Modification : EcoRI HindIII
Number of independent recombinants : 1.0 x 106
Titer : 1.3 x 108 pfu/ml
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen bacteria-free phage lysate.
Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Product Sheet
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