FT2 (ATCC® 77438)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

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Designations FT2
Species Homo sapiens, human
Depositors A Swaroop
Origin
thymus, fetal, 26 week
Vector
Tissue: thymus, fetal, 26 week
DNA: cDNA
Insert size range: > 1.0 kb
Genome Equivalent: 0.0
Biosafety Level 1
Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

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