rat kidney (ATCC® 77401)

Organism: Rattus norvegicus, rat  /  Clone Type: Library  /  Depositors: JC Edman

Designations rat kidney
Species Rattus norvegicus, rat
Depositors JC Edman
Origin
kidney, male
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Vector
Tissue: kidney, male
DNA: cDNA
Insert size range: 0.6 - 9 kb
Genome Equivalent: 0.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
Constructed after size selection of cDNAs. Internal EcoRI sites were methylated and inserts were cloned using EcoRI (8mer) linkers.
References

Morgan DO, et al. Insulin-like growth factor II receptor as a multifunctional binding protein. Nature 329: 301-307, 1987. PubMed: 2957598

Jeffrey C Edman, personal communication

Product Sheet
Product Sheet
Product Sheet